Preliminary results from high-resolution analyses of ultraviolet-absorbing and carbohydrate constituents in several pathologic body fluids.

Human urine, blood serum, cerebrospinal fluid, and amniotic fluid have been analyzed by high-resolution chromatographic systems under development at the Oak Ridge National Laboratory. These systems, or analyzers, consist of heated, high-pressure anion.exchange columns for chromatographic separation of the constituents and continuous-flow ultraviolet spectrophotometry or phenol-sulfuric acid colorimetry for detection of the separated ultraviolet-absorbing or carbohydrate components. Qua ntification of the resulting chromatograms has revealed significant differences in the concentrations of body-fluid constituents for the normal state and for various pathologic states such as hereditary nephritis, Lesch.Nyhan syndrome, gout, alkaptonuria, and maple syrup urine disease. Some changes in urinary chromatographic patterns resulting from nicotinic acid and allopurinol therapies have also been detected.